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Efektivitas Ekstrak Metanol Daun Salam (Eugenia Polyantha) Dan Daun Jeruk Purut (Cytrus Histrix) Sebagai Antijamur Pada Pertumbuhan Fusarium Oxysporum Image
Journal article

Efektivitas Ekstrak Metanol Daun Salam (Eugenia Polyantha) Dan Daun Jeruk Purut (Cytrus Histrix) Sebagai Antijamur Pada Pertumbuhan Fusarium Oxysporum

The Effectiveness of Methanol Extract of Bay Leaf(Eugenia polyantha) and Kaffir Lime Leaf (Cytrushistrix) as Antifungal on Growth of F. oxysporumEssential oils extracted from many plants have been investigated forbotanical pesticide source. There are several commercial pesticidescontaining essential oil. Kaffir lime containing citronella is known asantifungal material. Many research showed that bay leaf has antifungal andantibacterial activity. The objective of this research was to find out theeffect of metanol extracts of bay and kaffir lime leaves on F. oxysporumgrowth. The experiment was conducted from January to February 2008 atPhytopathology Laboratory of Indonesian Medicinal and Aromatic CropsResearch Institute (IMACRI). The experiment was consisted of 3activities : (1) Test of methanol extract of bay leaf and kaffir lime leaf onvegetative growth of F. oxysporum in solid media. Fungal colony diameterwas recorded each day. (2) Test of methanol extract of bay and kaffir limeleaves on generative growth of F. oxysporum in liquid media. Conidiaproduction and hifa weight were observed. (3) Test of methanol extract ofbay and kaffir lime leaves on inhabitation of conidia germination of F.oxysporum. The germination of conidia was observed at 0, 2, 4 hours aftertreatment. Experiment was designed using completely randomized designwith three replications. The result showed that methanol extract of bay leafinhibited the growth of F. oxysporum in solid media. The highest growthinhibition was 57.16% at 5% extract. In liquid media, methanol extract ofbay leaf decreased conidia production and hifa weight significantly.Methanol extract of bay leaf inhibited conidia germination. The percentageof inhibition of conidia germination was 84.67% at 3% extract. Methanolextract of Kaffir lime leaf inhibited the growth of F. oxysporumsignificantly. The percentage of inhibition was 95.6% at 5% extract. Inliquid media, methanol extract of kaffir lime leaf decreased conidiaproduction and hifa weight at all of concentration of extract. Methanolextract of kaffir lime leaf inhibited conidia germination. The percentage ofinhibition of conidia germination was 77.00% at 3% extract.
Pengaruh Komposisi Media Terhadap Pertumbuhan Kalus Dan Kadar Tannin Dari Daun Jati Belanda (Guazuma Ulmifolia Lamk) Secara in Vitro Image
Journal article

Pengaruh Komposisi Media Terhadap Pertumbuhan Kalus Dan Kadar Tannin Dari Daun Jati Belanda (Guazuma Ulmifolia Lamk) Secara in Vitro

West Indian Elm ( Guazuma ulmifolia Lamk.) is one of potentialplant producing tannin which is useful for controlling obesity. Tannin canbe produced through in vitro and this compound could be increased bycalli culture. The medium composition for calli induction was necessary toproduce the optimal calli. The aim of this research was to obtain themedium composition for calli induction through in vitro. Young leaves ofWest Indian Elm from glass house were used as explants. Murashige andSkoog (MS) medium enriched with B vitamin group was used as basicmedium. The experiments were arranged in completely randomized designin factorial pattern with ten replications. For calli induction, variousconcentration of 2,4-D (0.1; 0.3; and 0.5 mg/l) and its combination withBenzyl Adenin of 0.1 and 0.3 mg/l were used as treatments. Parametersobserved were calli diameter, structure, colour, fresh weight andperformance during culture. Analysis of tannin was conducted by usingdried samples both (in vitro and leaves from glass house) and thenextracted. The result showed that there was interaction between 2,4-D 0.3mg/l and Benzyl Adenin 0.1 mg/l on calli diameter (28.7 mm), the biggestcalli diameter after sub culture (31.9 mm), and fresh calli weight (5.02 g)eight weeks after treatments. Tannin content obtained from all of the invitro treatments (3.72 – 4.27%) was higher than tannin from leaves(2.24%).
Karakteristik Fisiologis Ralstonia Solanacearum Penyebab Penyakit Layu Bakteri Nilam Image
Journal article

Karakteristik Fisiologis Ralstonia Solanacearum Penyebab Penyakit Layu Bakteri Nilam

Karakteristik Fisiologis Isolat Sclerotium SP. Asal Tanaman Sambiloto Image
Journal article

Karakteristik Fisiologis Isolat Sclerotium SP. Asal Tanaman Sambiloto

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Detection of Phytoplasmas Associated with Kalimantan Wilt Disease of Coconut by the Polymerase Chain Reaction Image
Journal article

Detection of Phytoplasmas Associated with Kalimantan Wilt Disease of Coconut by the Polymerase Chain Reaction

Deteksi phytoplasma yang berasosiasi dengan penyakitlayu Kalimantan pada kelapa dengan reaksi rantaipolymeraseKelapa merupakan komoditi sosial kedua setelah padi di Indonesiadengan luasan areal lebih dari 3.6 juta ha pertanaman, ekuivalen dengansepertiga luas kelapa dunia, hal ini menjadikan Indonesia sebagai negaraprodusen kelapa terluas di dunia. Sekarang ini produksi dan produktivitaskelapa sangat rendah dan tidak stabil yang disebabkan oleh berbagai alasantermasuk serangan hama dan penyakit. Penyakit layu Kalimantan telahmengakibatkan kerugian yang besar pada pertanaman kelapa. Penelitiansebelumnya untuk mengetahui penyebab penyakit dilakukan denganmenguji bakteri, cendawan, virus, viroid dan patogen tanah sepertinematoda tetapi tidak ada yang secara konsisten berasosiasi denganpenyakit layu Kalimantan. Penelitian ini bertujuan untuk mendeteksi danmendiagnosa phytoplasma sebagai penyebab penyakit yang berasosiasidengan layu Kalimantan. Penelitian ini menggunakan dua metode untukmengekstraksi DNA yaitu metode CTAB yang biasanya menggunakannitrogen cair dimodifikasi dengan menghancurkan sampel tanaman padaCTAB yang dipanaskan, dan metode skala kecil. Hasil penelitianmenunjukkan bahwa kedua metode yang digunakan menghasilkan DNAyang sama baiknya untuk analisis PCR. Teknik nested PCR menggunakankombinasi primer P1/P7 dan R16F2n/R16R2 dapat membuktikan bahwapenyebab penyakit layu Kalimantan adalah phytoplasma. Teknik ini jugasecara efektif dapat mendeteksi phytoplasma dalam jaringan tanamankelapa yang sudah terinfeksi maupun yang belum menunjukkan gejalapenyakit. DNA phytoplasma dapat dideteksi pada 95 sampel dari 116sampel (81.9%) yang dianalisis. Berdasarkan jenis sample yang diperiksaternyata phytoplasma dapat dideteksi pada sample yang terinfeksi maupunyang belum menunjukkan gejala penyakit masing-masing 95.1% dan67.3%. Hasil penelitian ini mengkonfirmasi bahwa penyakit layuKalimantan disebabkan oleh phytoplasma.
Journal article

Pengaruh Perbandingan Air Kelapa Dan Penambahan Daging Kelapa Muda Serta Lama Penyimpanan Terhadap Serbuk Minuman Kelapa

Pengaruh Perbandingan Air Kelapa Dan Penambahan Daging Kelapa Muda Serta Lama Penyimpanan Terhadap Serbuk Minuman Kelapa Image
Journal article

Pemanfaatan Limbah Sagu sebagai Pengendalian Gulma pada Lada Perdu

Pemanfaatan Limbah Sagu sebagai Pengendalian Gulma pada Lada Perdu Image
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