The main constraint on soybean cultivation in acid sulfate tidal lands is the lack of P availability because it is bound by Fe. High rate of fertilizer applications often lead to high fertilizer residues that can be used for the following cropping using Arbuscular Mycorrhizae Fungi (AMF) inoculants. The research aimed to determine the effect of soybean culture techniques and AMF inoculants on growth and yield of soybean. The trial was arranged in a factorial randomized block design with two factors and three replications. The first factor consisted of four AMF inoculants from different sources, namely from rhizospheres of kudzu (Pueraria javanica), sorghum (Sorghum bicolor), corn (Zea mays) and soybean (Glycine max). The second factor was soybean culture tehniques, namely water saturated culture and dry soil culture. Seeds of soybean variety Tanggamus were grown in pots containing 5 kg soil derived from tidal land of Simpang Village, Berbak District, Tanjung Jabung Timur, Jambi. The results showed that there was a positive and significant interaction between the saturated soil culture and AMF inoculant from maize on the variabilities of P uptake (0.13 g/plant), P content in the plant (0.39%), relative efficiency of inoculants (72.8%) and relative efficiency of P uptake (133.3%), biomass dry weight (35.4 g/plant), the number of filled pods (106 pod) and seed dry weight of soybeans (27.6 g/tanaman). Dry soil culture and AMF inoculant from maize produced greater soybean root colonization. In overall, however, the saturated soil culture with AMF inoculant from maize gave the best growth and yield of soybean variety Tanggamus.