Downy mildew is an important disease in maize. Varietal resistance is considered the most practical for disease management. The research was aimed to obtain durable resistant to downy mildew on high-yielding maize varieties. The study was conducted in Kediri, East Java, in a downy mildew endemic area. Ten new high-yielding maize varieties were tested. The treatmens was arranged in a randomized block design with three replications. Seeds of each variety were sown in a 5 m x 4 m plot, at a 75 cm x 20 cm plant spacing consisted of 120 plants/plot. At 10 days after planting (DAP), the crops was fertilized with urea, SP-36, and KCl at rate of 100 kg, 100 kg, and 100 kg/ha, respectively. The second and third fertilizer applications were done at 30 and 45 DAP using 100 kg urea/ha. Results showed that varieties Bima-5, HJ 21 Agritan, Bima-14 Batara, and Bisi-19, which were previously highly resistant to downy mildew, showed low resistance durabilities and became susceptible to the disease, with the disease intensities ranging from 62 to 77.2%. Conversely, Bima-3 Bantimurung, Bima-20 URI (STJ 109), Bima 5 Sayang, and Lagaligo (composite variety) indicated more durable resistance with the disease intensities ranging from 13.5 to 20.3%, as compared to the susceptible variety Anoman (100%). The effect of durable resistant to downy mildew was also shown on the maize yield and 1000 seeds weight.