Analysis of STI Mobile Services Utilization For STI and HIV-AIDS Prevention by KaraokeGuide of Graha Mitra Partnership in Mororejo Village Jepara; Jepara district has one ofthe biggest hotspots that Karaoke tour Pungkruk Beach in Mororejo that high risk oftransmission of STIs and HIV - AIDS . Health Department along with Graha Mitra hold STIMobile for karaoke guide with the purpose of expanding the range of services . But a visit tothe service is still very low . This study aims to analyze the utilization of STI mobile services in the prevention of STI and HIV - AIDS by guiding karaoke .It was quantitative methods with cross-sectional approach. and data was collected for 35 karaoke guides. Results showed that 62,9 % of respondents never used STI mobile services. Results of bivariate analysis showed variable knowledge of STI, attitudes and needs of the service had a significant association with the utilization of STI mobile services. Multivariate indicate that the variable needs of the service are significant variables affect the utilization of STI mobile services.