Progo Hulu River is the main river in Progo watershed, Temanggung Regency. It was estimated that human activities have some important contributions in its water quality degradation, for example housings/settlement, agricultural, industry, etc. This research was aimed to analize Progo Hulu River which focused on (1) river water quality, (2) some factors that cause the different effects of each segment concentration, and (3) water quality status for every segment. The river water quality was observed in 12 sampling-points and then it compared with water quality standart according to the Government Act No. 82/2001. This research also describes water quality status used STORET and Pollution Index method based on Ministry Decree No. 115/2003. As result, Fecal Coliform become a dominant parameter that caused pollution in first class water quality, whereas Ammonia and Fecal Coliform caused pollution in second, third, and fourth class water quality with a main pollution source from domestic and ranch wastes. Besides, water quality status showed it has been modaretely polluted in first class water quality status and lightly- modaretely polluted in second, third, and fourth class water quality status.