Domestic wastewater have high concentration of COD. The increasing amount of domestic wastewater can affect the quantity of wastewater in receiving water which exceeds the carrying capacity of environment. Thus, will cause negative effect in environmental quality. On this research, the wastewater was treated by using Dual Chamber Microbial Fuel Cells (DCMFCs). Microbial Fuel Cells is a device which is used to convert chemical energy to electricity using microorganism as catalyst assistance. In order to achieve maximum COD removal and electrical discharge, COD concentration and graphite rod electrode surface area has been varied. COD concentration varied on 400 mg/l, 800 mg/l, and 1200 mg/l . While the graphite rod electrode surface area varied on 25 cm2, 49 cm2, and 74 cm2. DCMFCs reactor operated in 2 sequences, acclimatization on 14 days and reactor running process on 35 days. The optimum COD removal and power density result produced by 800 mg/l COD concentration and 25 cm2 elctrode surface area.