The land in Gedangsari Subdistrict area composes of limestone. Many local people consume drinking water from wells that contain high levels of calcium. Many people suffer from urolithiasis. This study aimed to describe calcium or Ca(OH)2 distribution in the well water and explain its relation with urolithiasis incidence. This study was conducted in Gedangsari Subdistrict, Gunung Kidul District from July to November 2013. The study was cross sectional confirmed with titration test in laboratory. Samples were 94 wells of 3,849 well population as selected randomly. Criteria of sample selection included wells used for drinking by the population aged older than 30 years already, with less than 15 meter of depth. Laboratory test of Ca (OH)2 level was conducted by titration. Suspect urolithiasis was clinically diagnosed by doctor and data analysis used chi-square test. Results showed relation between water hardness and urolithiasis (RP = 2.27), although statistically not significant. In conclusion, there was no relation between mineral water consumption, age, and length of stay with urolithiasis incidence in Gedangsari Subdistrict, Gunungkidul District.