. Bamboo is natural composite material which is built from many types of cells. Fibers cells which are bundled become vascular bundles have highest contribution toward the strength of bamboo split among others. Besides the amount of vascular bundles, the bamboo split strength is also determined by chemical component of cell walls and its position (internodes/node). This research reported that the tensile strength has positive correlation with the amount of vascular bundles. If the amount vascular bundles increase, then the tensile strength also increases. The increasing of lignin contents in cell wall proportionally and quadratically increases the tensile stress of internodes and node of bamboo split, respectively. This research did not found the maximum stationer point of quadratic equation for lignin content effect toward bamboo split tensile strength, thus the tensile strength always increase along with the lignin content in this research range (<33%). Correlation value between tensile strength and ratio of alpha cellulose to holocellulose is high which prove that alpha cellulose highly contribute to the tensile strength even though best subset regression procedure could not identified this phenomenon because of its autocorrelation occurrence.