Depik, Rasbora tawarensis is an endemic species in Lake Laut Tawar, Indonesia, and this species has been listed as threatened species. Reproductive biology data is one of the important information to strategise conservation plan. This paper reported the spawning ground of the depik, hence this paper is contributing the additional importance information on the reproductive biology of R. in relation to provide comprehensive our understanding on the reproductive biology of this species. The study was conducted during July to November 2009 in Lake Laut Tawar. A total of 13 spawning grounds were detected in the study where the locations are distributed in five villages namely, five locations in Mendale, two locations in Kelitu, two locations in Gegarang, three locations in Bewang dan one location in Pedemon. However, only four sites remained active in the dry season i.e. two sites in Kelitu and two sites in Gegarang villages.