Treatment of cancer such as surgery, radiotherapy and chemotherapy have many side effects such as cardiotoxicity, hepatotoxicity, and immunosuppressant, therefore selective cochemopreventive agent is needed. Awar-awar (Ficus septica) is a traditional medicinal plant that is known as a potential cancer chemopreventive agent. The purpose of this study was to determine the cytotoxic effect of ethanolic leaves extract of awar-awar (EFs) against 4T1 and MCF7/HER2 breast cancer cells; WIDR colon cells cancer, HeLa cervix cells cancer, and Vero normal cells. The cytotoxic test was performed by MTT assay. The parameter obtained from the cytotoxic test was IC50. Selectivity index was determined from IC50 ratio of cancer cells to normal cells Vero. The results showed that EFs has a cytotoxic activity on cancer cell line with IC50 61,2 µg/mL on 4T1; 48 µg/mL on MCF7/HER2; 122,4 µg/mL on Hela; 75,9 µg/mL on WiDR; and 394,8 µg/mL on Vero. It can be concluded that EFs has high selectivity on various cancer cells with selectivity index more than 3.