A total of 35 grape accessions and 3 reference cultivars were used to investigate the genetic polymorphism and relationships among Turkey and other European grape accessions by ampelographic characterization. Total of 74 ampelographic characteristics were identified for 38 genotypes. Ampelographic data were collected two vegetation periods. The characteristics of the vines were defined and measured according to OIV descriptors. In this study, three synonym varieties (100% similarity) were identified: 'Cabernet Sauvignon' and 'Cabernet Franch'; standard grape variety 'Merlot' and no.12 genotype (Merlot), and 'Sèmillion' analyzed as a standard grape variety and no. 1 'Sèmillion' genotype. Based on similarity rate of grape varieties, highest similarity ratios were found between 'Yuvarlak Razakı-Siyah Gemre' with 93%, 'Yuvarlak Razakı-Siyah Gemre' and 'Şika' with 91%, 'Moiseylative-Hafızali' with 90% and 'Kırmızı Şam-Pembe Gemre' genotypes with 89%. In principal component analyses graph, 'Müşküle', 'Buca Razakı', 'Moiseylative', 'Kırmızı Şam', 'Cardinal', 'Yuvarlak Razakı', 'Hafızali', 'Siyah Gemre' and 'Şika' were grouped together.