Soil and water quality are the factors that determine the brackishwater ponds total production in Lamongan Regency, East Java Province. This research aimed to determine direct effect and indirect effect of soil and water quality on total production of brackishwater ponds policulture of white shrimp and milkfish productivity in Lamongan Regency. The research was conducted in brackishwater ponds of Glagah, Karangbinangun, Turi, Sukodadi, Karanggeneng, and Maduran Subdistricts, Lamongan Regency. Soil quality was defined as independent and exogenous variables; water quality as an intermediate, dependent and endogenous variables; and total production as dependent and endogenous variables. Characteristics of soil and water quality as well as the total production is known through descriptive statistics application, while the effect of soil and water quality on total production is known through path analysis applications. The results of path analysis showed that 15 soil quality variables analyzed was only 2 variables that affect on total production, namely: pHKCL and Al, and 11 water quality variables analysed only 2 variables that affect on total production namely: DO and pH. pHKCL of soil has significant effect with direct effect of 0.314 on total production, while Al has hightly significant effect with direct effect of -0.426 on total production. DO and pH of water have direct effect of -0.367 and -0.245 on total production, respectively.