Antibiotics have become a necessity to fight infections. These antibiotics are produced by microorganisms and industrially they are produced in fermentation tanks by using desired microorganisms. The fermentation media contains carbon source, nitrogen source and trace elements along with anti-foaming agents. The carbon source best suited for efficient production of bioactive compounds by microorganisms includes glucose and lactose sugar. By changing the source of obtaining glucose for media preparation the antibiotics production can be made more cost efficient and also more sustainable. Lignocellulosic waste materials includes plant dry matter which is easily available and this can be converted into cellulose and further into glucose by various physical, chemical or biological methods. With increasing demands of antibiotics, lignocellulose can be considered as a reliable raw material for their production.