Improved rice varieties at areas that have problems with the availability of phosphorus (P) is very important. Pup1 locus, thelocus containing genes that play a role in the P uptake, has been well mapped and some markers for selection have beendeveloped. Based on previous studies, BC2F8 lines have been obtained from crosses of Dodokan × Kasalath (DK), Dodokan ×NIL-C443 (DN), Situ Bagendit × Kasalath (SK), Situ Bagendit × NIL-C443 (SN), Batur × Kasalath (BK), and Batur × NIL-C443(BN). This study aimed to evaluate the BC2F8 lines at molecular level as well as their yield potential. The molecular researchwas conducted from November 2013 to June 2014 at ICABIOGRAD, Indonesia and IRRI, Philippines, whereas the field trialswere conducted at Taman Bogo Field Station, Lampung and a farmer's land at Sukabumi, West Java. Molecular analysisdemonstrated that all of the BC2F8-Pup1 lines contained Pup1 locus. However, three lines (B5-SK5, B9-SN2, and C9-BN2)containing the Pup1 locus were found in heterozygotes condition. All of the Pup1 lines still retained the genome compositionof the parent, except for B7-SK7, C10-BN3, and C11-BN4. B1-SK1, B2-SK2, B3-SK3, B4-SK4, B6-SK6, B9-SN2, C4-BK4, C7-BK7,and C12-BN5 Pup1 lines have yield more than their recurrent parents (Situ Bagendit or Batur) in areas with either low orenough available P conditions. B6-SK6 dan C12-BN5 Pup1 lines have yield more than their recurrent parents and checkvarieties (Inpago 7 dan Inpago 8) in area with low available P condition. These lines could be used for multilocation trial.