Keragaman Jumlah Salinan Transgen Galur T0 Padi Kultivar Nipponbare Berdasarkan Analisis QPCR Dengan Penanda Gen HptII

Aqwin Polosoro • Wening Enggarini
Journal article Jurnal AgroBiogen • Desember 2016

Unduh teks lengkap
(Bahasa Indonesia, 8 pages)

Abstrak

The development of transgenic crop using Agrobacterium tumefaciens produces different inserted transgenes, whether copy numbers or location in the plant genome. The research was performed to detect chimeric phenomena based on the transgene quantity analysis in tillers of a clump and of some clumps which were derived from the same calli. CsNitr1–L gene and hptII gene as a marker on a binary plasmid pCAMBIA1300 was transformed into the Nipponbare rice genome using A. tumefaciens strain LBA 4404. Molecular analysis was carried out on three tillers of each four clumps of Nipponbare transgenic T0 generation (events number 1, 2, 3, and 4) and four groups of T0 clump derived from one callus. Three T0 clump samples were collected from each of the four groups of T0 clumps. The results of qPCR analysis showed that the transgene copy numbers of tillers which were derived from one T0 clump were the same. qPCR analysis also discovered that not all plants from one callus demonstrated the same transgene copy numbers. This implies that each T0 rice clump which grows from the transformed calli was needed to be split in the acclimatization step so that the uniform T1 seeds would be obtained.

Metrik

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Jurnal

Jurnal AgroBiogen

Jurnal AgroBiogen semula bernama Buletin AgroBio yang diterbitkan pada tahun 1996 hingga 2004. Se... tampilkan semua