Muridae Famili that live in Java includes 10 genera and 22 species. Muridae Famili that often found inhuman residents are Rattus norvegicus ("riul" rats), Rattus tanezumi (asian house mouse) and Musmusculus ("piti" mouse). Nematode (roundworm) is rodents parasiticon. Some species of these worms canalso infest humans (zoonosis). This study conducted in 2010 aims to determine the type of parasiticnematodes that infest Muridae Famili (rats and mice) in human residents in the Banjarnegara District.Thisstudy used a descriptive research design and laboratory research type. The data was collected throughcapturing Muridae using live traps placed for consecutive 3 days. Result this study, Muridae capturing tookplace Kutabanjarnegara Semampir village with trap success 11.77% and 7.77% respectively. Muridaespecies that obtained were Rattus tanezumi, and Mus muscull, Rattus tiomanicus. While nematodesobtained were Syphacia muris from Kutabanjarnegara village 15,38% and semampir village 31,25%,Nippostrongilus Brasilliensis from Kutabanjarnegara 5,76%, also founded form Semampir villageGongylonema neoplasticum 31,25%, Masthoporus muris 33,33% and Tikusnema javaense 33,33%meanwhile nematodes reported to be zoonotic were Syphacia muris and Gongylonema neoplasticum.