Folikel Antral Ovarium Pada Pemberian Antibodi Monoklonal Bovine Zona Pelusida 3 Sebagai Kandidat Imunokontrasepsi

Milatun Khanifah
Journal article Jurnal Ilmiah Kesehatan • 2016 Indonesia

Unduh teks lengkap
(Bahasa Indonesia, 9 pages)

Abstrak

Overpopulation is still an importance issue in developing countries such as Indonesia. On the other side of the contraceptive method that is currently relied upon to handle such problems have many deficiencies that promote efforts to suppress a population of less than optimal. Development of contraceptive methods such as the use of non-hormonal targets Monoclonal antibodies bovine imunokontrasepsi ZP3 as a candidate material needs to be done. This study aims to determine the effect of Mab-bZP3 as candidate materials immunokontrasepsi against ovarian histology, namely the number of antral follicles are normal and atretic. True experimental research was conducted on 36 animals model, there were consist of three control groups and three treatment groups, respectively were analyzed on day 5th, 10th, and 20th after the treatment. The control groups were given an injection of 50 mL of Phosphate Buffer Saline and the treatment groups were given an injection of Mab bZP3 50 mL. There was no significant evidence on discrepancy effect of Mab bZP3 toward average number of normal antral follicles (p-value 0.715) and atretic antral follicles (p-value 0.604). There was no significant evidence on discrepancy effect of Mab bZP3 toward average number of atretic antral follicles (p-value 0071) between the times observation on each of the control and treatment groups. However, There was significant evidence on discrepancy effect of Mab bZP3 toward average number of normal antral follicles (p-value 0.036) between the times observation on their respective control and treatment groups. The control group were examined on day 5th, the only significant different with the control group and the treatment group were examined on the 20th day. While the average number of normal antral follicles lowest for the control group were examined on day 20, but no significant evidence on discrepancyin the treatment groups were examined on the 20th day. These results indicate that Mab bZP3 as a candidate imunocontraceptive material has no effect on follicles development, in terms of the regulation of apoptosis and FSH receptors on the granulosa cells. Reversiblility of contraceptive methode, as demonstrated by evidance that no significant disperancy effect of Mab bZP3 toward average number of antral follicles between control and treatment groups on day 20th.

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Jurnal Ilmiah Kesehatan

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