Journal article
Stenotrophomonas Maltophilia, Bakteri Resisten Merkuri pada Limbah Pertambangan Logam
—Mercuric-resistant bacterium isolate coded 22 was previously obtained from metal mining (tailing) wastewater samples. From the total 24 bacteria which had been isolated, isolate 22 exhibited the highest resistance toward high concentration of Hg. In this paper it is reported results of 16S rDNA-based identification of isolate 22 as well as its growth and resistance characteristics when growing on Mercuric containing Nutrient Broth medium. Isolate 22 is closely related to the bacterium, Stenotrophomonas maltophilia (identity 99% and query covered 90%). Isolate 22 grew very well in the NB-Hg medium containing Hg concentration of 50 and 100 ppm. Increasing Hg concentration clearly lengthened lag phase although cell quantity (culture turbidity, OD600) at the initial phase were the same.
Keywords: isolate22, Hg, identification, sequencing, 16S rRNA gene