Introduction: Comfort is a state of psychical wellbeing, safe and avoid from the anxiety and fear. The temperature measurement is essential to identify the changes in body temperature on the health status of pre-school age children. Comfortably temperature measurements is with minimizing anxiety and fear in pre-school age children. The purpose of this research was to determine the effect temperature measurement of infrared tympanic membrane thermometer to the comfort of pre-school age children. Method: The study design was a “Quasi-experimental” to asses the effect of independent variables on a dependent variable. The study of population was pre-school age in kid polyclinic in Rumah Sakit Umum Daerah Cibabat. That there is used a consecutive sampling with sample size of 21. Result: Results there is a characteristic effect of age with a level of comfort with the use of infrared tympanic membrane thermometer, there was no correlation with the level of comfort gender, there was no correlation experience of being treated with a level of comfort. Discussion: Suggestion expected to be used as a reference in subsequent studies, ie about comfort in temperature measurement in pre-school children by adding confounding variables and measure the sensitivity of the tool.