Toxicology may be defined as the study of harmful / poisonous effects of drugs and other chemicals. The present study was aimed to determine LD50 aqueous fraction of Impatiens balsamina L by acute toxicity study as per OECD guideline 425.Rats were sequentially administered all the fraction in single dosage of 2000 mg/kg body weight and 5000 mg/kg of body weight. Female Sprague Dawley 8-12 week and weight between 140-170 gram were used. One rat was treated with aquadest that served as the control. All the animals were individually studied for mortality, wellness parameters, food consumption,water consumption, body weight. Histopathological examination was done on the liver and kidneys. No mortality and no significant changes were observed in wellness parameter, body weight, food consumption, and water consumption at 2000 and 5000 mg/kg of body weight. Histopathological examination of fraction treated groups showed lesion of hepatic cells. The result of this study showed that LD50 aqueous fraction of Impatiens balsamina L was greater than 5000 mg/kg of body weight, which is classified as mild toxic.The aqueous fraction of Impatiens balsamina L is safe to use as indicated by the high LD50 value.