Indonesia has many kinds of plants which can be used as antioxidant sources. This study is focused on antioxidant activity of Cincau Perdu leaves extract (Premna oblongata Miq.) by1,1-Diphenyl-2-picrylhydrazyl (DPPH) methods. Premna oblongata Miq.leaves was extracted using n-hexane, ethyl acetate and methanol. The IC50 value of methanol extract as the highest activity as antioxidantwas 20.01 μg/mL. The extract which had the highest antioxidant activity were fractionated by accelerated column chromatography and were earned 6 combination fractions. The antioxidant activity of each combination fractions were tested by DPPH methods. Fraction-5 showed the highest activity as antioxidant with IC50 23.51 μg/mL. The compounds of the active fractions were flavonoid, glikon, saponin and tanin.