High blood cholesterol level is one of the coronary heart disease risk factor causing high mortality in developed country. Bay leaf containing flavonoids can inhibit HM-CoA reductase enzyme.Objective: The aim of this study is to explore bay leaf infusion effect and optimal dose in reducing blood cholesterol level in dyslipidemic rat model. Method: Experimental complete randomized design was done using 25 male Wistar rats weighing 200-300 gram at Pharmacology Laboratory Maranatha Christian University in January-October 2011. After dyslipidemic condition was induced with high fat diet and PTU (propil thiouracil) 0,01 % for 2 weeks, simvastatin, 2 mL bay leafe infusion was given to each of them in 5, 10, and 20 % concentration orally for 2 weeks. Blood total cholesterol level was measured after high fat diet and PTU and after bay leaf infusion/ simvastatin treatment. Data was analyzed with ANOVA, continued with LSD with α= 0,05. Result: Bay leaf infusion in 5, 10, 20% lowered the blood total cholesterol level (p< 0,05). There was no difference between these bay leaf infusion concentrations and simvastatin each other in lowering the level (p > 0,05). Conclusion: It was concluded that bay leaf infusion in 5, 10, 20 % concentration had the same effect in lowering blood total cholesterol level in dyslipidemic rats and the potency was same as simvastatin.