Introduction: Lingzhi mushroom (Ganoderma lucidum) is the herbal medicine that have antiinflammatory, hepatoprotective, hypoglycemic, hypotensive, and hypolipidemic activities. Lingzhi mushroom contain triterpenoid that reported has hypolypidemic activity by cholesterol syntesis inhibitor.Objectives: Determine the triglyceride profile on hypercholesterolemi rats by ethanol extract of lingzhi mushroom.Methods: There are 20 rats that were divided 4 groups. Group 1 was given standard diet. Group 2,3,4 were given high-fat diet then given standard diet and aquadest 2.5 ml/200mg body weight (for group 2), diet standar and ethanol extract of lingzhi mushroom 200, 400 mg/kg body weight (for group 3, 4).Results: After treatment, levels triglycerides in all groups decrease. The decreasing triglyceride levels did not significantly between groups 3, 4 with group 2 and group 1 (p>0.05).Conclusion: Administration ethanol extract of lingzhi mushroom did not affect on the level triglyceride in hipercholesterolemi rats.