Metrik

  • visibility 0 kali dilihat
  • get_app 0 downloads

Development and Evaluation of Elisa Kits Based on Proteomics TECHNOLOGY for Detection and Semi-Quantification of Meat Species Adulteration

Manal Mohamed Elsayed Ahmed, Walid Nazmy, Asmaa Noureen
Diterbitkan 2022

Abstrak

Purpose: This study aimed to develop ELISA kits for the detection of meat species adulteration in raw and processed meat and evaluate its specificity and sensitivity. Method: We identify the unique peptide markers based on the proteomics approach. Subsequently, we prepared specific antisera for beef, pork, horse, sheep, chicken and developed ELISA kits. Results: Evaluation of the developed kits in testing crooked reference sample mixtures revealed that the developed ELISA kits showed 100% specificity with no cross-reactivity detected for the non-target species. In terms of sensitivity, the developed ELISA kits were able to consistently detect pork in the sample mixtures and horses at levels down to 5% w/w. Although sheep and chicken were detected at levels as low as 1% w/w. The beef-specific ELISA test showed greater sensitivity. Conclusions: It is concluded that the developed ELISA kits are a promising tool but further studies are still required for validation of the developed kits and comparing its results with the commercially used kits before entry into the production phase.

Full text

 

Metrik

  • visibility 0 kali dilihat
  • get_app 0 downloads