Over the past century, there has been a dramatic increase in worldwide research.However, these rapid increases are also arising problems or difficulties faced bythe university students. Therefore, this research was carried out to reveal thetypes of those difficulties faced by students of STKIP Pacitan PGRI in solving thetwo-factor experiment tests in the design of experiment subject and the factorswhich cause these difficulties. The researcher opted the qualitative descriptivedesign to the qualified research findings. Moreover, the samples, 15 students,were randomly taken from the total population or 93 students who attended thelectures. The technique of collecting the data employed both written test andinterview of the researched subject. Based on the research findings, the studentswere difficult in determining the source of variance (SV), the degrees of freedom(df), the Expected Mean Square (EMS), the F value. Briefly speaking, the studentswere difficult in the process of calculating. While, the factors that caused thedifficulties were in distinguishing the state of R, drawing a conclusion from allthese factors, determining the number of r (replicate), determining the removedpart from EMS, being careless in equalizing the line of numerator anddenominator candidate, being unable to develop formulas, and careless in thecalculation.