This paper aims to analyze the overall impact of transformational leadership style directly and indirectly on employees' job performance mediating by work satisfaction and moderating by organizational culture. Furthermore, to find the influence of transformational leadership on employees' job performance mediating by work satisfaction and moderating by organizational culture within educational institution in Indonesia. This is a survey-based research study. Primary and secondary data were used in this study. Primary data were gathered via questionnaire formal and informal interview. Sample size is (n = 320); therefore, descriptive statistics, validity reability analysis and structural equation modelling analysis have been used. The overall results support that transformational leadership style has a significant positive impact on employees' job performance as well as mediating by work satisfaction and moderating by culture at selected educational institution in Indonesia. Transformational leadership style is the most important factor for achieving work satisfaction goals. This study fulfils an acknowledged necessity of learning the impact of transformational leadership, work satisfaction, culture on employees' job performance.