The main fokus of this research was to find out the effect of motivation and ability on employee's performance at PNPM Mandiri Perdesaan Office of Central Java Province. The respondents in this study were all employees there were 25 employees. Statistic analysis that used to test the hypothesis was multiple linier regression, and to be counted with SPSS for Window program. Due to the study, it was found that there were significant positif effects of ability and motivation on employee's performance at PNPM Mandiri Perdesaan Office of Central Java Province. So it is important to improve ability and motivation in order to improve employee's performance.