Typhoid/paratyphoid disease is a major problem in Indonesia. Management of therapy including the use of immunomodulator must be developed continuously. In this case Phyllanthus niruri L is immunomodulator that has been useful to increase the animal and human immunity. The purpose of this study was to know the influence of Phyllanthus niruri L on the Salmonella infection focusing on spleen bacterial colonies. The experiment was designed with post test-only control group to 18 BabiC Mice infected by Salmonella typhimurium divided into one control group and two experimental groups which have given Phyllanthus niruri L of 3x0.125 mg/day (P1) and 3x0.25 mg/day (P2) orally. Statistical analysis was used Oneway ANOVA test. The research result showed that treatmen P1 had significant influence to decrease spleen bacterial colonies compared to control group.