Leptospirosis is a public health problem all over the world, especially in tropical and subtropical countries. International Leptospirosis Society (ILS) states that Indonesia is the third-highest country in the world in terms of Case Fatality Rate (CFR). The purpose of the study is to figure out the epidemiological overview of leptospirosis case in Boyolali Regency based on variables of person, place, and time. The type of the study is descriptive using quantitative methods. The sample of the study is all leptospirosis cases in the districts in Boyolali consisted of 47 cases. The result of the study shows that leptospirosis case occurs more in adults of age group 26-45 years (38,3%), are male (70%), and work as farmers (44,7%). Leptospirosis case is most prevalent in 2014 and the highest incident cumulative (0,6) happens in March 2014 and 2015. The most prevalent case of leptospirosis in Boyolali occurs in Nogosari district with 16 cases (34%). Counseling on the prevention of leptospirosis transmission needs to be conducted so that people can avoid the danger of leptospirosis.