: Asphyxia neonatorum was an emergency baby condition that can not breathe spontaneously and regularly, so it can decrease oxygen and increase carbon dioxide their lead to bad consequences in further life. Role of the nurse in helping the newborn, especially infants with severe asphyxia is to be able to realize good coordination, quality of service standards and nurses equipped with the knowledge and service processes should be given equipped with the right attitude. purpose of research was to determine the relationship between knowledge and attitudes of nurses with handling severe asphyxia in newborns. The research method in this study uses survey research methods analytic cross sectional design. Samples in this study uses total sampling as many as 15 people. Research results using statistical analysis chi-square test value obtained p = 1.000> α = 0.05. Research Conclusion there was no correlation between knowledge and attitudes of nurses with handling severe asphyxia in newborns in NICU room at RSUP Prof. Dr. R. D. Kandou Manado.