Audio Media Analysis Of Behavior Change Use of Personal Protective Equipment (PPE)in Health Laboratory Officer in Banjar; The number of occupational accidents in Indonesiatend to rise, in 2010 there were 98711 cases and 2011 there were 99491 cases of occupational accidents. Workplace accidents also occurred on clinical laboratory worker at Banjar hospital. Clinical laboratory workers is part of health care workers who always associated with specimens of patients who have a risk of getting infected by various diseases, especially when there is no discipline in the use of personal protective equipment (PPE). The type of this research is a quantitative analytical study with quasi-experimental research design (Quasi - experiment design). Number of samples are 38 health laboratory workers in Banjar region. Data analysis includes univariate and bivariate using paired t-test. The results of the study : There are significant differences of posttest II independent t-test between control group and experimental group, in the variables of knowledge, attitude, and practical use of PPE. There are significant differences of paired t-test for experimental group between pretest and posttest II, in the variables of knowledge, attitude, and practical use of PPE.Audio media effectively to changes the behavior of the use of PPE in health laboratory workers in Banjar.