Head of households behavior in using toilet at Tawiri Village, Teluk Ambon regency, Ambonmunicipality; Behavior of using toilet is the most effective and simple to prevent several kind of disease such as diarrhea, typhoid, cholera, and dysentery.. The purpose of this study is to examine factors influencing toward head of households behavior in using toilet at Tawiri Village Teluk Ambon Regency Ambon Municipality. This research is an explanatory research with cross sectional design. Study population consisted of 93 head of households chosen by using a simple random sampling. The data was collected by interview and observation. The findings showed that as much as 72,0% head of households have used toilet and as much as 28,0% head of households haven't used toilet. There are any correlation between toilet availability, knowledge of using toilet, attitude towardusing toilet, communities leader support, health officials support, and religious leader support. While based on logistic regression analyze, the majority factor was influenced toward head of households behavior in using toilet at Tawiri Village, Teluk Ambon Regency is religious leader support (OR=19,116).