Tooth Brushing Behavior on the Elementary School Students of Grade V and VI in Sumberejo Sub-District; One of preventions for dental caries is by keeping mouth cleanliness by brushing the teeth properly and correctly. The age of elementary school is the ideal time to train a child's motoric ability, such as teeth brushing behavior. The aim of the research is to identify the factors influencing teeth brushing behavior on the elementary school students of grade V and VI in Sumberejo Sub-Distict, Tanggamus District. The type of this research was explanatory research with cross sectional approach. The research subjects were the elementary school students of grade V and VI in Sumberejo Sub-Distict, and the number of samples was 285 students. The data analysis was conducted in univariate, bivariate with chi square and multivariate with logistic regression. The bivariate analysis results on the variables associated with tooth brushing behavior are the parents role (p=0.008), teacher's role (p=0.007). The results of logistic regression test show that the most influencing variable on tooth brushing behavior in the elementary school students of grade V and VI in Sumberejo Sub-Distict, is teacher's role (OR=1.934). It means that good teacher's role has the effect on students to have good tooth brushing behavior twice as much as poor teacher's role.