Cotton production areas in Indonesia are arable land with lack of wateravailability. The aim of this study was to obtain cotton lines tolerant todrought using PEG-6000 at germination stage. The experiment wasconducted at the Seed Testing Laboratory in Indonesian Sweetener andFiber Crops Research Institute, from April to June 2012. Treatments werearranged in a Split Plots design with two replications. The main plot wasPEG-6000 (-3 bar) and without PEG-6000 (water), while the subplot was13 cotton lines and Kanesia 14 variety. Seed cotton was treated with 80%Mancozeb fungicide dose of 2g/kg seed before sowing. Parametersobserved were germination percentage, shoot and root length, shoot androot weight, ratio of root/shoot length, and drought susceptibility index.Seedling was counted as normal if its length more than 0.5 cm. PEG-6000treatment gives very significant effect on the decline on seed germination,shoot and root length, shoot and root weight. Otherwise, ratio of root/shootlength was higher in the PEG-6000 than without PEG-6000 treatment.Response of cotton lines to drought which calculated with a droughtsusceptibility index were different among parameter observed. Meandrought susceptibility index of all parameters showed that none of cottonline tolerant to drought (S < 0,50) was achieved from the study. Therewere eight moderately drought-resistant lines (0, 50 < S < 1,0) achievednamely 03002/12, 03006/1, 03008/7, 03008/24, 03012/17, 03014/12,03017/13, and 03017/15 which more resistance than Kanesia 14.