Keragaman Morfologi, Pertumbuhan, Produksi, Mutu Dan Fitokimia Keladi Tikus (Typonium Flagelliforme Lodd.) Blume Asal Variasi Somaklonal

Sitti Fatimah Syahid
Journal article Industrial Crops Research Journal • September 2008

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(Bahasa Indonesia, 6 pages)

Abstract

Morphological variation, growth, production, quality andfitochemistry of rodent tuber (Typonium flagelliformeLodd.) Blume derived from somaclonal variationRodent tuber (Typonium flagelliforme Lodd.) Blume is one ofmedicinal plant which is used for healing cancer. Conventionally, the plantwas vegetatively propagated, therefore. Its genetic variation is narrow. Anattempt to increase the genetic variation of plant was conducted usingsomaclonal variation (calli culture) and resulted the normal planlet in vitro.But, the plant morphology, growth, production, quality and fitochemistryvariation have not been evaluated yet. The aim of the research were toobtain the morphological variation, growth, production, quality andfitochemistry of rodent tuber from calli culture seeds. Experiment wasdone at the Indonesian Medicinal and Aromatic Crops Research InstituteGreen house from November 2005 to January 2007. The plants materialused were seeds of rodent tuber from calli culture compared with seedsfrom in vitro culture. They were planted in the polibag (20 cm x 30 cm)which is contained soil and cow manure (2:1) and maintained until ninemonths. The experiment was arranged in completely randomized designwith sixteen replications. The parameters observed were morphologicalcharacter and growth component at six months and rhizome production atnine months. Further more, quality and fitochemical variation and rhizomewere also analyzed. The result showed that visually, morphologicalcharacter of those plant observed were same in leaf, stem and rhizome.Growth component (number of leaves, length and width of leaves) of seedsfrom calli culture were same with in vitro and conventional but plantheight was different. Conventional seeds produced the greatest tillers butrhizome production was lower than seeds from calli and in vitro. Watersoluble extract from in vitro seeds was higher than calli culture andconventional seeds. Steroid compound was detected in seeds from calliand in vitro. On the otherhand, flavonoid and triterpenoid were not found.The experiment showed that application of somaclonal variation on rodenttuber only increase the quality and fitochemical variations but not on plantmorphology.

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Industrial Crops Research Journal

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