Determination of parasitic nematode Aphelenchoides sp.causing leaf blotch disease of sambiloto (Andrographispaniculata)Leaf blotch disease by parasitic nematode Aphelenchoides sp. havebeen reported infected and causedleaf drops on sambiloto (Andrographispaniculata (Burm. f.) Wall. ex Nees) (Acanthaceae). Experiments ofspecies determination of the nematode have been carried out inlaboratorium, greenhouse and fields of IMACRI during the year 2005-2006. Identification were done by observing its typical symptoms of theinfected plants caused by the nematode and its nematode morphologicalcharacteristics microscopically on the semi-permanent preparats fixed byTAF (triethanolamine formaldehyde) solution. Typical symptoms of leafblotch were began as chlorotic vein-deliminated areas which later changedto light brown, then dark brown and finally black; or sometimes purplishunder field condition. Microscopically, the female was slender, 0.46 –0.70 mm long and 15 µm width in average, the vulval region cited about2/3 of the body length sized from anterior part; spear slender, 10 µm longwith small and distinct basal knobs; large metacorpus occupying ¾ ormore of the width of the esophagus; lip region almost continuous withbody contour; the tail tip was elongate-conoid ending in a simple bluntspike. The male was abundant and essentially similar to size and shape ofthe female; tail arcuate through 45º to 90 when relaxed, with a simpleblunt terminal spine; spicules rose-thorn shaped. Those describedsymptoms and morphological characters mentioned above were the typicalcharacters of the species Aphelenchoides fragariae (RITZEMA BOS,1891) CHRISTIE 1932. Detection of species A. fragariae was the firstreport in Indonesia; and sambiloto as the natural host of A. fragariae wasthe first report in Indonesia and Internationally.