. The potential of biochar derived from oil palm kernel shell to be used as a soil amendment seems promising for sustaining oil palm plantation in marginal land. This study was conducted to investigate the effects of three biochar dosages (50; 100; and 150 gram) in combination with 75% NPK-Mg dosages per seedling on the growth of oil palm seedling, nutrient uptake and carbon sequestration in the nursery stage. A field experiment was conducted at PT Astra Agro Lestari Tbk, Central Kalimantan in March 2015- April 2016. Experiment was arranged in a completely randomized block design (CRBD) with five treatments and twenty replications i.e: (i) 100% standard dosage NPK-Mg per seed (control); (ii) 75% standard dosage NPK-Mg per seed; (iii) 75% standard dosage NPK-Mg + 50 g biochar per seed; (iv) 75% standard dosage NPK-Mg + 100 g biochar per seed; and (v) 75% standard dosage NPK-Mg + 150 g biochar per seed. The best vegetative growth performance of oil palm seedlings in Lithic Hapludults soil was shown by the application of 75% standard dosage of NPK-Mg fertilizers in combination with 150 gram biochar per seed. This treatment increased height (33.9%); leaf number (36.2%); stem diameter (28.9%); length and width of the leaf (22.6 and 33.3%), dry weight of oil palm seedlings, especially roots (65.2%), CEC (17.2%), and organic C (26.9%). This treatment also showed the highest absorption of N in leaves of oil palm seedlings, but no differences were observed for P and K uptakes compared to the treatment of 100% dosage of NPK-Mg fertilizer. On the basis of these results, the 75% dosage of NPK-Mg combined with 150 g of biochar per seed can be recommended for use on oil palm seedlings.