Eksplorasi Bakteri Pendegradasi Insektisida Klorpirifos Di Lahan Sayuran Kubis Jawa Barat

Eman Sulaeman • Asep Nugraha Ardiwinata • Mohamad Yani
Journal article Indonesian Soil and Climate Journal • 2016 Indonesia

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(Bahasa Indonesia, 10 pages)

Abstract

. Chlorpyrifos insecticide is one of insecticide that mostly used by farmer to control many kind of pests, but the use of this insecticide continuously have effect to environmental damage, decrease of land quality, and human health. Improving quality of land contaminated with insecticide can be done with bioremediation by using microorganism activities. This research was started by taking soil samples in cabbage crop land at Cisarua, Pacet, and Lembang districts, spread in Nutrient broth (NB) medium that contained chlorpyrifos, and grown isolates were purified on Nutrient agar medium. The purified isolates were tested their ability to degrade chlorpyrifos insecticide, and then identified by 16S rRNA molecular identification. The result shows that 6 isolates were obtained from soil samples from Cisarua, Pacet, and Lembang districts. They grow on contaminated medium and have ability to degrade chlorpyrifos insecticide about 8,66 to 50,63%. The best of 3 isolates that had the highest ability to degrade chlorpyrifos insecticide were C3NP1 (39,67%), P5NP (50,63%), and L9NP (44,98%). The resulted of molecular identification shown that isolate C3NP1 has 99,80% similarity to Pseudomonas monteilii, isolate P5NP has 95,60% similarity to Bacillus cereus, and isolate L9NP has 92,70% similarity to Pseudomonas sp.

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Indonesian Soil and Climate Journal

Jurnal Tanah dan Iklim contains the results of research in the field of land and climate from res... see more