Fertile women farmers are risky of suffering decrease of cholinesterase activity due to pesticide exposure. This study aimed to analyze relation between pesticide exposure and the exposure agent to cholinesterase activity of fertile women workers at Kedunguter Village. This study used cross-sectional design on 94 fertile women farmers in 2015. Data was collected by observation, interview and cholinesterase test. Data analysis used chi-square test and analysis results showed a significant relation between pesticide types, working time, the use of gloves, hand-washing behavior to cholinesterase activity of fertile women farmers. Analysis results of this study showed that variable working time had the highest odds ratio (OR) score (OR = 14.072), so the variable working time is the most dominant variable in influencing cholinesterase enzyme. This study suggests that fertile women farmers should work not more than six hours per day.