Antioxidant activities are responsible for beneficial health effect and correlated wellwith polyphenols and flavonoids content. Red algae is considered as rich source of natural antioxidant. The study objective was to investigate phytochemical screenings and antioxidant activity of ethanol fractions Euchema spinosum from south Bangka waters in fresh algae (FA), dry algae (DA) and algae product (AP) by DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Extraction by acidic 70% ethanol for 24 hr and evaporated using rotary evaporator. Dry extract fractionated with ethanol : n - hexane ( 2 : 3 ) and dried ethanol fraction.The phytochemical screenings showed these fractions presence of flavonoid, alkaloid and triterpenoid compounds. The antioxidant activity was superior to ethanol fraction of AP with IC50 value 333.66 μg/mL, FA 418.32 μg/mL and DA 472.14 μg/mL. Based on the results obtained, it can conclude that the algae product ethanol fraction may be valuable natural antioxidant sources.