A drug therapy can alter the pharmacokinetic profiles and biodistribution patterns of radiopharmaceuticals. Glucocorticoids are pharmaceutical drugs for anti-inflammatory by preventing phospholipid release and decreasing eosinophil action. To achieve an optimum diagnostic outcome, this research was focused on pharmacokinetics interaction and biodistribution pattern between two kinds of Glucocorticoids drugs i.e. dexamethasone and prednisone with 99mTc- MDP using animal model Mus musculus stock Swiss. 99mTc-MDP has been developed as radiopharmaceutical for bone imaging in nuclear medicine. Mice were divided into three groups, which were treated with dexamethasone by oral administration for 5 days continously, treatment with prednisone by oral administration for 3 days continously and without treatment (control). Pharmacokinetics interaction was conducted by injecting 200mL 99mTc-MDP intravenously administrated using a dose 1 mCi/mL. Biodistribution pattern was conducted by injecting 200 mL99mTc-MDP intravenously administrated using a dose 1 mCi/mL. After 3 hours after intravenousinjection of 99mTc-MDP each of these groups of animals were killed with chloroform and then dissected. Radioactivity of blood samples and selected organs were weighed and counted by using single channel analyzer. The results of pharmacokinetics study showed that the elimination half-life of animal model that given with dexamethasone and prednisone are 4.61 h and 4.63 h more faster than control animals (20.67 h). The results of biodistribution study showed that uptake of 99mTc- MDP in bone using animal models decreased which were given dexamethasone and prednisone compared to normal animals, which following results 3.53 ± 0.49%, 3.47 ± 0.5% and 11.54 ±4.36% (control).