Mahkota Dewa (Phaleria Macrocarpa) sebagai Antinefrotoksisitas “Dewa Penyelamat” dalam Penurunan Efek Samping Cisplatin

Zulharini S, Meirizky • L.R, Annishfia • Hidayah, Siti Nurul • Iman, Naisbitt

Abstract

Cisplatin is one of the most widely used chemotherapeutic agent in cancer treatment, however it possess series of harmful adverse effects, most notably, nephrotoxicity. Due to that reason, a natural chemopreventive agent is needed to minimize cisplatin's toxicity, namely, Mahkota Dewa fruit (Phaleria marcocarpa) extract. This research aim to determine anti nephrotoxic effect of mahkota dewa fruit on Vero cells, model of renal cells. Cytotoxic assay of mahkota dewa's extract and cisplatin both single and combination was determined using MTT assay on HeLa cells and Vero cells. The cytotoxic assay resulted that IC50 value of cisplatin and Mahkota Dewa to HeLa cells were 18µM (5,4 µg/mL) and 845 µg/mL, respectively, whereas the IC50 value of cisplatin and Mahkota Dewa to Vero were 80 µM (24 µg/mL) and 730 µg/mL, respectively. The results indicated that cisplatin was more cytotoxic to HeLa cell in comparison to Vero cell. Combination treatment of mahkota dewa's extract at 183 µg/mL and cisplatin 284 µM showed increased viability of Vero cells. Therefore, combination treatment of cisplatin and mahkota dewa are able to decrease nephrotoxicity of cisplatin to renal cells.

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Pekan Ilmiah Mahasiswa Nasional Program Kreativitas Mahasiswa - Penelitian 2013

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