Nowadays, in the newest era of biology science,biology has progressed and developed. One of the developments marked by the need for Biological Sciences in the field of bioengineering.Thus, it is importance for under graduates have a competence in technical analysis of molecular biology. One course that teaches techniques related to Molecular Biologyis Technical Analysis of Molecular Biology.Based on the results of the needs analysis survey conducted teaching in Technical Analysis Course Molecular Biology course. there are nobooks, handouts, modules, and media interactive video that used in Technical Analysis Course Molecular Biology class. More students use journals and other supporting materials associated to the course. Based on these results, it is necessary to the development the interactive video, in which mainly discusses about the principles and basic techniques in molecular biology analysis, to facilitate students to understand and practice the basic principles of Technical Analysis in Molecular Biology.Interactivemedia has developed based on the research results of analysis of genetic variation buffalo(Bubalusbubalis) at Bima. The development follows the model of the development model Kempand Dayton(1985) which subsequently conducted product trials by media experts, expertmaterial, andasmallgroup trials as evaluative measures developed product.