Diagnosis Schistosomiasis dengan Metode Dot Blot

Samarang Samarang • Made Agus Nurjana • Malonda Maksud • Phetisya Pamela Frederika Sumolang • Gunawan Gunawan
Journal article Media Penelitian dan Pengembangan Kesehatan • 2017 Indonesia

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(Bahasa Indonesia, 6 pages)

Abstract

The diagnosis of schistosomiasis to detect secretory excretory antigen (AgES) Schistosoma japonicum by ELISA method, has been developed since 2012 through laboratory tests with good result, so it is necessary to do field test. Based on this the researchers conducted field tests, of schistosomiasis with dot blot in Napu, Poso district, Central Sulawesi Province. This study was conducted for nine months (March-November 2014) with cross sectional design. This study aims to determine the sensitivity and specificity of ELISA developed (dot blot). Fecal and blood surveys were conducted in communities in selected endemic villages, using simple random sampling method, with a total sample of 325 people. Furthermore, the blood serum tested using ELISA with dot blot technique. The sensitivity and specificity of the blot technique was calculated againts the results of microscopic examination (gold standard). Field test result showed the sensitivity and specificity of dot blot was 74% and 78%. Conclusion dot blot technique can detect Schistosomiasis on a laboratory scale or on a field scale.

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Media Penelitian dan Pengembangan Kesehatan

Media Penelitian dan Pengembangan Kesehatan (Health Research and Development Media) is an open ac... see more