Port Health Office Clas II Semarang has conducted control of Ae. aegypti larvae using larvadisa Temephos 1%. Temephos (Abate 1SG) has been used since 1980 for the eradication program of Ae. aegypti larvae. The purpose of this study is to determine the resistance status of Ae. aegypti larvae against temephos in perimeter and buffer area of Tanjung Emas Port Semarang. This research was conducted using experimental research design, posttest only with control group. Population of the research were larvae of Ae. aegypti collected from the study area and samples test larvae were used of Ae. aegypti third and early fourth instars larvae which were maintenance of the first generation of Ae. aegypti. The advance test used concentration 0,625 mg/l; 0,31 mg/l; 0,15 mg/l; 0,078 mg/l; 0,038 mg/l. Based on mortality percentage of Ae. aegypti larvae in perimeter area (96%) indicates on tolerant criteria. While the mortality percentage of Ae. aegypti larvae in buffer area (68%) indicates on resistant criteria. Statistic test using Kruskall-Wallis test showed that there is a significant difference in the average of mortality Ae. aegypti from perimeter and buffer area with p value 0,001. Post-hoc test using Mann-Whitney test showed that there is a significant difference in the average of Ae. aegypti against temephos at concentration 0,31 mg/l; 0,15 mg/l; 0,078 mg/l; 0,038 mg/l, while there is no significant difference of mortality Ae. aegypti against temephos in concentration 0,625 mg/l. The use of temephos still possible in perimeter area, but larvasida rotation is necessary in buffer area.