Residues of pesticide can cause harmful effect for the environment and public health. Pesticide poisoning in the human body can cause a decrease Cholinesterase levels in the blood. Cholinesterase examination can be done by checking blood Cholinesterase activity. The purpose of this study is to determine the factors associated with pesticide poisoning among farmers in Jati village, District Sawangan. The research used Cross sectional design with total sample of 92 farmers. The variables of this research are blood Cholinesterase levels of respondents, age, frequency of spraying, the level of knowledge, work period, personal protection tools and the length of farmers spraying pesticides. The research location is in Jati village, Sawangan subdistricts, Magelang districts. The research shows that there some variables which give significant results. Those are: Frequency Spray ( PR = 13,791; 95% CI = 3,551 to 53,557 ), Knowledge Level ( PR = 1,668; 95% CI = 1,059 to 2,628 ), Work Period ( PR = 4,958; 95% CI = 2,322 to 10,583 ), and Old Work ( PR = 11,110; 95% CI = 4,323 to 28,556 . While the farmers have yet to the use of protectivethemselves with complete.The conclusion of this research is the risk factor of the level of knowledge, the frequency of spraying, the completeness of personal protective equipment used, work period, and length of farmers work have an influence on the incidence of pesticide poisoning with a decrease in blood Cholinesterase levels of farmers in Jati village, Sawangan subdistricts, Magelang districts.