Irradiation is one of the main activities in SIT. Currently, its needed a method for producing a male sterile by male pupae irradiation in large quantities and produce sterile adultin high emergence. This study was aimed to analyze the influence of density variations of Aedes aegypti male pupae in gamma ray radiation media of 70 Gy to the rate of adult emergence. This type of research is experimental design with post test only control group. The sample was used in this study as many as 5,400 male pupae. The method was used by irradiated male pupae with different densities of each tube vial (d=2 cm), ie 60, 120, 180, 240 and 300, each of density had 5 repetitions and control (not irradiated). After irradiation, it was observed adult emergence rate. The results showed that an increase in the density of irradiated pupae inversely proportional to the adult emergence rate. Based on the results of ANOVA test with p=0,01, there was influence between the density of irradiated pupae to adult emergence rate. Based on the results of Tukeys test with p=0,01 indicated that adult emergence best rate was in the density of 120 individuals. Based on these findings, irradiation should be done with pupae density was 120 individuals.