Perkembangan Koloni Primer Embryonic Stem Cell (Esc) Mencit Pasca Vitrifikasi Inner Cell Mass (Icm)

Boediono, Arief • Rinendyaputri, Ratih
Journal article Indonesian Bulletin of Health Research • September 2013 Switzerland

Abstract

Application or the basic research of the stem cells is continously conducted to explore their potential, particularly the use of stem cells in health. Cryopreservation technology is needed to maintain availability of stem cell. This study was aimed to observe the development of murine embryonic stem cell (ESC) primary colonies post-vitrified inner cell mass (ICM). Inner cell mass (ICM) was derived from blastocysts of murine embryos of female Swiss webster mice using pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Inner Cell Mass (ICM) was isolated from blastocysts by immunosurgery. Inner Cell Mass (ICM) were divided into two groups: the control and vitrified ICM. Both groups were cultured and observed on the ICM attachment (attachment rate/AR), the primary formation of ESC colonies (primary colony/PC) and diameter of primary colony ESC which grouped into small, medium and large. The results showed that AR and medium diameter's group of primary colony ESC were lower (p<0.05) in vitrified ICM than control ICM. However the percentage of PC and diameter of primary colonies ESC in small and large groups were not different compared with control (p>0.05). This study shows that vitrification for cryopreserving ICM can be used as an alternative source of ESC. Key words: blastocyst, inner cell mass, ICM, vitrification Abstrak Submit : 30-01-2013 Review : 08-02-2013 Review : 11-03-2013 revisi : 26–03-2013 Aplikasi maupun penelitian dasar untuk menggali potensi sel punca khususnya pemanfaatan sel punca di bidang kesehatan terus dilakukan. Teknologi kriopreservasi dibutuhkan untuk menjaga ketersediaan sel punca. Penelitian ini bertujuan mengamati perkembangan koloni primer embryonic stem cell (ESC) mencit pasca vitrifikasi inner cell mass (ICM). Inner Cell Mass (ICM) diperoleh dari embrio blastosis mencit betina Swis webster menggunakan pregnant mare's serum gonadotropin (PMSG) dan human chorionic gonadotropin (hCG). ICM diisolasi dari embrio blastosis menggunakan metode immunosurgery. ICM yang diperoleh dibagi menjadi dua kelompok yaitu ICM kontrol dan ICM vitrifikasi. Kedua kelompok dikultur serta dilakukan pengamatan terhadap tingkat perlekatan (attachment rate/AR) ICM, tingkat pembentukan koloni primer ESC (primary colony/PC) dan diameter koloni primer ESC yang dikategorikan menjadi 3 kelompok yaitu kecil, sedang dan besar. Hasil menunjukkan bahwa ICM vitrifikasi mempunyai persentase AR dan diameter koloni primer ESC pada kelompok sedang lebih rendah (p<0,05) terhadap ICM kontrol. Namun persentase PC serta diameter koloni primer ESC kelompok kecil dan besar pada ICM vitrifikasi tidak menunjukkan perbedaan (P>0,05) terhadap kontrol. Penelitian ini menunjukkan bahwa vitrifikasi dapat digunakan untuk kriopreservasi ICM sebagai sumber ESC. Kata kunci : blastosis, inner cell mass, ESC, vitrifikasi

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Journal

Indonesian Bulletin of Health Research

Buletin Penelitian Kesehatan (Indonesian Bulletin of Health Research) is a quarterly, open access... see more