Background: Community participation was necessary for culturinglocal strain of Bacillus thuringiensis H-14 in coconut medium and adoption this bioinsecticide to control Anopheles sundaicus. This study aimed to evaluate community participation for culturing local strain of B. thuringiensis H-14 using coconut media and spreading the results of inoculation against Anopheles sundaicus larvae. Methods: The study design was a quasi-experiment. Reseach was conducted in February-November 2007 in Kampung Laut, Cilacap. Samples were 30 person which were choosed purposively. The sample criterias were at least 15 years of age and have a pond. Community participation was evaluated for 6 times using checklist. results: Results showed that all respondents (> 80%) could have on every element of 9 elements properly. Ninety percent of respondents could prepare coconut independently and made local strains of B. thuringiensis H-14 inoculation in coconuts properly (96. 67%). The density of An. sundaicuslarvae was decreased by 80–100% after 1 day spreading with culture of local strain of B. thuringiensis H-14. conclusion: Communities could participate independently for culturinglocal strain of B. thuringiensis H-14 in coconuts to control An. sundaicus larvae. The inoculation capable todecrease An. sundaicus larvae density 80–100% after 1 day. Further research was recomended to calculate cost benefit and cost effectiveness.