Background: Iron deficiency anaemia is the most common nutritional problem of pregnant women in Indonesia. The existing program/intervention has been implemented namely supplementation of iron pills for mothers at the third semester of pregnancy. This study aims to compare the effect of single and multiple micronutrients supplementation of iron and iron plus vitamin C. Methods: Type of the study is study experimental with the design of community intervention, implementing pre and post trials. Subjects were pregnant women at the second semester of pregnancy. Haemoglobin level was examinedby Cyanmet hemoglobin. Data analysis was done through independent and paired t-test. Results: showed that there were statistically signifi cant difference between the intervention and control group (p = 0.001). The haemoglobin level of the intervention group increased by 0.91 grm%, while the control group was 0.43 grm%. The proportion of anaemia amongpregnant mothers declined after intervention from 80.95 to 42.86 at the intervention group. Similarly, the proportion of anaemia of the control group reduced from 80.95% to 71.43% after the intervention. Conclusion: intervention with multiple micronutrients of Fe combined with vitamin C has more effect in changing the haemoglobin levels of pregnant mothers.Recommendation: Fe tablet administration should still be accompanied by vitamin C to help boost hemoglobin levels. Required for controlled administration of iron tablet, along with vitamin C so that the effect can be seen.